Antibiotic yields anxiolytic

Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the potent antibiotic taromycin

1. Experimental methods 2. Strains and plasmids used in this study (Table S1) 3. Deduced functions and sequence comparison of the tar genes (Table S2) 4. Schematic diagram of the tar gene cluster direct cloning (Figure S1) 5. Restriction mapping of pCAP01-tar and its derivatives (Figure S2). 6. Results of heterologous expression of the tar gene cluster in S. coelicolor M1146 (Figure S3) 7. Resu...

Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin A.

Recent developments in next-generation sequencing technologies have brought recognition of microbial genomes as a rich resource for novel natural product discovery. However, owing to the scarcity of efficient procedures to connect genes to molecules, only a small fraction of secondary metabolomes have been investigated to date. Transformation-associated recombination (TAR) cloning takes advanta...

Pseudopheochromocytoma induced by anxiolytic withdrawal

BACKGROUND Symptomatic paroxysmal hypertension without significantly elevated catecholamine concentrations and with no evidence of an underlying adrenal tumor is known as pseudopheochromocytoma. METHODS We describe the case of a female patient with paroxysmal hypertensive crises accompanied by headache, vertigo, tachycardia, nausea and altered mental status. Previously, she was treated for a ...

Sedative-Hypnotic and Anxiolytic Drugs

The primary use of sedative–hypnotic and anxiolytic drugs is to encourage calmness (anxiolytics or sedatives) or to produce sleep (sedative–hypnotics). All people are subject to states of emotional tension and uneasiness. For otherwise healthy individuals, these occasions are usually sufficiently mild and short that pharmacological intervention is unnecessary. However, at times the symptoms of ...

Effects of tillage methods on barley yields